| Sheif Life: | 2 Years |
|---|---|
| Storage: | Cold Dry Place |
| Deliver Time: | 7-10 Days |
| Samples: | Available |
| MW: | 626.50464 |
| Purity: | >98% |
| Samples: |
|---|
| Customization: |
|---|
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CAS No.: 39450-01-6
EINECS No.: 3.4.21.64
Form: Lyophilized White Powder.
Source: Mutated gene from Tritirachium limber, Expressed in recombinant yeast.
Specific Activity: ≥34U/mg dry weights
Package: 100mg/Bottle, 1g/Bottle (Standard) or 1-200 gram Customer packing.
Storage Conditions:Store at 4 to -20ºC recommended.
Mutated Proteinase K powder is stable at room temperature in 3 years.
Expiration : Three years if kept below 4ºC.
Molecular Weight: 29,300 D
QC Assays:
Activity Assay and Unit Definitions:
One unit is defined as the amount of enzyme that will liberate 1 μmol of tyrosine per minute at 37º C, pH7.5.
DNase Activity: none detectable enzyme activity with λ DNA after 6 hours incubation at 37ºC.
RNase Activity: none detectable of ribnuclease activity after 16 hours incubation at 25 º C
Protein Purity: over 99% (Native-PAGE and SDS-PAGE assay)
|
Description |
White powder |
Complies |
|
Identification |
IR |
Complies |
|
|
HPLC |
Complies |
|
Heavy metal |
≤10ppm |
5ppm |
|
Pb |
≤3ppm |
1.5ppm |
|
Hg |
≤0.1ppm |
0.05ppm |
|
Cd |
≤1ppm |
0.2ppm |
|
Loss on drying |
≤0.5% |
0.12 |
|
Residue on lgnition |
≤0.1% |
0.03 |
|
Single impurity |
≤0.5% |
0.12 |
|
Total impurity |
≤1.0% |
0.29 |
|
Total bacteria |
≤1000cfu/g |
<1000 |
|
Yeast and Mould |
≤100 cfu/g |
<000 |
|
E.coli/25g |
Absent |
Absent |
|
Salmonella/25g |
Absent |
Absent |
|
Assay |
≥99.0% |
99.4% |
|
Conclusion |
Conforms with USP/EP standards |
|
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