96 Tests Magnetic Bead Viral DNA/Rna Extraction Kit
Purchase Qty. / FOB Price:
$ 2.83
Min. Order: 10,000 Pieces
Shanghai, China
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All
CE, FDA, ISO13485
Nucleic Acid Extraction Kits
Biological Diagnostics
bioteke
Wuxi
Bioteke Corporation (Wuxi) Co., Ltd.
Wuxi, Jiangsu, China
Business Type: Trading Company
Product Details
Quick Details
96 Tests Magnetic Bead Viral DNA/Rna Extraction Kit
All
CE, FDA, ISO13485
Nucleic Acid Extraction Kits
Biological Diagnostics
bioteke
Wuxi
Product Details
Product Name
Magnetic Bead Viral DNA/RNA Extraction Kit
Specification
96 tests/kit
Intended Use
It is used to isolate and purify high-quality viral DNA / RNA from tissue, blood, serum,
plasma, lymph fluid, acellular body fluids, cell culture supernatants or various virus
preservation solutions.
Test Principle
During the nucleic acid extraction process, the magnetic bead adsorption principle is used
to adsorb, transfer, and purify nucleic acids through special magnetic beads to
automatically complete the nucleic acid extraction.
Applicable instrument
Suitable for AU1001-96 automatic nucleic acid extraction instrument
Sample requirements
Fresh samples. Experiment as soon as possible after sample collection. It can be stored temporarily at
2-8 ° C. If long-term storage, it should be stored at -20 ° C or -80 ° C.
Instructions
1. Reagent preparation (40 mg/mL concentration of Proteinase K solution preparation)
1.1 Add 1 mL of deionized water into each 40 mg proteinase K dry powder to get a proteinase K solution
with concentration of 40 mg/mL.
1.2 Add 500 uL of deionized water into 20 mg of proteinase K dry powder to get a proteinase K solution
with concentration of 40 mg/mL.
1.3 Mix upside down and about 10 times to make sure the dry powder completely dissolved. The solution
can be stored at 2-8 ° C for short-term use. If long-term storage, keep at -20 ° C. The number of repeated
freeze-thaw cycles shall not exceed 5 times.
2. Sample preparation
Tissue samples: Take 10 ~ 30 mg of liquid nitrogen and grind to a fine powder, transfer to a 1.5 mL
centrifuge tube, add 200 μL of 1 X PBS buffer to resuspend, and then proceed to the next step.
Sample Preservation Tube (with virus): no need to deal with, proceed directly to the next step
3. Pre-packaged deep well plate preparation
3.1 Carefully tear the plastic film of the pre-packaged deep-well plate and keep the deep-well plate
stable.
3.2 Add 200uL of prepared sample to the No. 1 deep well plate (96 tests). If sample volume is not
enough, add PBS buffer. Then add 25 uL of proteinase K solution (concentration 40 mg/mL).
3.3 Put this deep well plate into the extractor smoothly, and then insert the stirring sleeve into the card
slot.
4. Procedure for setting up the extractor
"Temperature setting": set the lysis temperature to 90 ° C and the elution temperature to 56 ° C. After saving the settings, click "Run" to start the experiment.
5. After the experiment is completed, transfer the DNA/RNA solution in the No. 6 deep-well plate to a
clean nuclease-free centrifuge tube for direct downstream experiments or to be stored at -20 ºC
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