| Type: | Biological Buffer |
|---|---|
| Appearance: | Powder |
| Grade Standard: | Reagent Grade |
| Form: | White Powder Crystallization |
| pH Value: | 2.5-4.0 (25ºC, 4m in H2O) |
| The Purity: | 99.95 |
| Samples: |
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| Customization: |
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Audited Supplier Introduction:
Trihydroxymethylaminomethane hydrochloride is referred to as Tris-HCl. Tris-HCl buffer is not only widely used as a solvent for nucleic acids and proteins, but also has many important uses.
Application:
Tris-HCl was used for protein crystal growth at different pH conditions. The low ionic strength of Tris-HCL buffer can be used for the formation of intermediate fibers of lamin in C.elegans. Tris-HCl is also one of the main components of the protein electrophoresis buffer. "In addition, Tris-HCL is an intermediate in the preparation of surfactants, vulcanization promoters, and some drugs." Tris-HCl was also used as a titration standard.Buffer solution preparation:
Tris-HCl buffer solution with a concentration of 6.057g/L and a pH value of 8.8 was prepared by using Tris-hcl HCl as the solute and deionized water as the solvent.
Advantages:
1. Trihydroxymethyl amino methane hydrochloride has good water solubility, high solubility in water, and liquid clarity;
2. High purity, up to 99%, stable performance;
3. Do not precipitate with some metal ions (such as calcium and magnesium);
4. The ionic strength of Tris-HCl buffer is low;
5. Tris-HCl is more effective than the traditional phosphate buffer in the enzymatic assay.
Chemical properties:
White crystals.
Purpose:
1.The pH of all buffers was temperature and concentration dependent. For Tris buffer, pH increased by approximately 0.03 units per 1°C increase in temperature and decreased by 0.03 to 0.05 units per 10-fold dilution of pH.
2.For use in biological buffers
3.It is used as an organic synthesis intermediate
| Point of melting: | 150-152 °C |
| Boiling point: | 225ºC[at 101 325 Pa] |
| Density of density: | 1.05 g/mL at 20 °C |
| Pressure of vapor: | 0Pa at 20ºC |
| Storage conditions: | room temp |
| Degree of solubility: | H2O:4 Mat 20 °C,clear,colorless |
| form: | White powder crystallization |
| PH value: | 2.5-4.0 (25ºC, 4M in H2O) |
| Coefficient of acidity (pKa): | 8.1(at 25ºC) |
| Acid-base indicator discoloration ph range: | 7.0 - 9.0 |
| Solubility of water: | SOLUBLE |
| Sensitivity: | Hygroscopic |
| Maximum wavelength (λmax): | λ: 260 nm Amax: 0.12λ: 280 nm Amax: 0.08 |
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