Biological activity:
CHAPS, a derivative of Cholic acid, is a zwitterionic detergent that solubilizes membrane proteins. CHAPS is used to stabilize various protein-DNA complexes and can retain the biochemical activity of proteins in solution.
Purpose:
CHAPS is a nondenaturing zwitterionic detergent used in membrane biochemistry. It can be used to dissolve membrane proteins as well as to interrupt protein-protein interactions. CHAPS has a low micelle molecular weight (6,150) and a high critical micelle concentration (6-10mM), and thus can be removed from the sample by dialysis. It is also suitable for solubilizing proteins in isoelectric focusing and two-dimensional electrophoresis. CHAPS is commonly used for nondenaturing (urea-free) isoelectric focusing and has been found to yield excellent resolution for certain subcellular preparations and plant proteins. "Concentrations between 2-4%(w/v) are typically used for isoelectric focusing gels."
A non-denaturing zwitterionic detergent for membrane biochemistry that can be used to dissolve membrane proteins and to interrupt protein-protein interactions; Also? Suitable for solubilizing proteins in isoelectric focusing and two-dimensional electrophoresis; Often used for non-denaturing (urea free) isoelectric focusing; RNA and DNA were extracted without rnase and dnase activity
zwitterionic surfactant, CHAPS is a nondenaturing zwitterionic detergent, protein lysate for solubilizing membrane proteins and cleaving protein-protein interactions.
| Point of melting: |
156-158°C (dec.) |
| Density of density: |
1.01 g/mL at 20 °C |
| Index of refraction: |
1.6900 (estimate) |
| Storage conditions: |
-20°C |
| Degree of solubility: |
H2O: 50 mg/mL at 20 °C, clear and colorless |
| form: |
White powder crystallization |
| Acid-base indicator discoloration ph range: |
6 |
| Solubility of water: |
Soluble in water (10 mg/ml). Soluble in DMF and hot methanol. |
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